QBM

Chapter 13: DNA Purification, Agarose Gel Electrophoresis, & DNA Visualization - ANSWER The Genetic Code: - ANSWER - Genetic info. of all living organisms is contained within the sequence of nucleic acids (purines and pyrimidines) - For most organisms, the genetic info. is contained in DNA) or (RNA) in certain viruses Genome: - ANSWER - The complete set of the nucleic acids containing the genes for an organism - 5 million bp in E. coli (4300 genes) - 3 billion bp in human genome (~21,000...

QBM exam| Questions Solved with Verified Correct Answers | Latest Update Two pieces of DNA can be joined together by Klenow fragment. DNA ligase. restriction enzymes. Primase. DNA polymerase. - ANSWER DNA ligase Which of the following is NOT a type of "next generation" DNA sequencing? - ANSWER Maxam Gilbert sequencing

What is another name for a Western Blot? - ANSWER Immunoblot Technique that allows one to detect and quantify proteins that react with a specific antibody. - ANSWER Western Blot Protein identification on SDS-PAGE is based on comparison to markers of what? - ANSWER Known molecular weights

What are the first three steps for starting a project? - ANSWER 1 ) Choose 2) Read literature 3) Obtain DNA sequence Once you obtain the DNA sequence in the beginning of a project, BLAST will allow what? - ANSWER "Basic Local Alignment Search Tool" - To search for homology (duplication events) - Can predict protein, molecular weight, pI, and extinction coefficient - Predict the secondary/tertiary structure, transmembrane helices, ubiquitation sites, etc

What is the best representation of DNA/protein if you could see it? - ANSWER Molecular surface model OSHA - ANSWER Occupational Safety and Health Association - Must inform employees of potential hazards MSDS - ANSWER Material Safety Data Sheet - Lists chemical's physical and chemical properties, toxic, carcinogenic - Describes proper handling Biosafety levels - ANSWER BSL-1 = minimal hazards BSL-2 =potential hazardous organisms, requires PPE - QBM LAB BSL-3 = pathogenic organisms that ...

Protein Expression - ANSWER Why can't proteins be synthesized formation acids via ORGANIC CHEMISTRY? - ANSWER - Most simple way to synthesize proteins - Can only synthesize about 50-70 amino acids - Useful for short peptides; Ex: alpha helixes - Proteins may misfold In vitro (cell free): - ANSWER - Uses mRNA & ribosomes: mRNA --> protein directly in tube via ribosomes (or human cell extract)

Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? - ANSWER miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? - ANSWER Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? - ANSWER Miniprep.

Which of the following refers to manipulating a piece of DNA and putting it into a plasmid for the purposes of gene studies, protein expression, and gene therapy? - ANSWER Recombinant DNA technology What type of bond is found in a protein that connects individual amino acids? - ANSWER peptide Employers must inform employees and train them in the safe handling of potentially harmful chemicals. This is required by - ANSWER OSHA

In vitro protein expression - ANSWER -Fast -Expensive -Low yield Prokaryotic and Bacterial cell Protein expression - ANSWER -Takes up plasmid fast - high yield - inexpensive -Expression proteins toxic to EUK cells -disadvantage: inclusion bodies and improper folding Eukaryotic Protein expression - ANSWER -Yeast and plant cells - large proteins - folded properly -Tedious DNA manipulation