Plasmid vectors Study guides, Class notes & Summaries

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

AMCB 2023 Test Questions with Correct Answers To verify DNA purity we have to: - Answer-Measure absorbance at 260 nm and 280 nm. The addition of proteinase K in the buffer ATL is to: - Answer-Disrupt the cell envelope to lyse cells. The genomic DNA that we extract from cells (of any kind) is polar and sticky. This property is useful for purification purposes. Which of the following surfaces is likely to be used, with good efficiency, to purify DNA. - Answer-A glass rod/bar To quantify...

AMCB 2023 Test Questions with Correct Answers To verify DNA purity we have to: - Answer-Measure absorbance at 260 nm and 280 nm. The addition of proteinase K in the buffer ATL is to: - Answer-Disrupt the cell envelope to lyse cells. The genomic DNA that we extract from cells (of any kind) is polar and sticky. This property is useful for purification purposes. Which of the following surfaces is likely to be used, with good efficiency, to purify DNA. - Answer-A glass rod/bar To quantify ...

Craniofacial Biology, Exam 1 with Practice Questions and Answers Name two specific purposes for cloning foreign DNA into plasmid vectors ANSWER protein drug production (like insulin) and gene therapy Briefly describe the cloning process ANSWER -restriction endonuclease digests and inserts the DNA into a vector with ligation - introduce newly ligated DNA into host cell (like E coli) -identify bacteria that have taken up the proper + vector Where to RE come from and what do Restriction ...

recombinant DNA - ANSWER DNA produced by combining DNA from different sources genetic engineering - ANSWER the transfer of a gene from the DNA of one organism into another organism, in order to produce an organism with desired traits biotechnology - ANSWER A form of technology that uses living organisms, usually genes, to modify products, to make or modify plants and animals, or to develop other microorganisms for specific purposes. gene cloning - ANSWER The production of multiple copie...

Microbiology Exam 2 practice Tests 208 Questions with Verified Answers Which of the following statements is correct? A) For every amino acid, there is one codon. B) A codon may signify more than one amino acid. C) For every codon, there is one amino acid. D) All of these are correct. - CORRECT ANSWER For every Codon, there is one amino acid The sigma factor of RNA polymerase is necessary for A) maximum transcription of the gene. B) termination of the mRNA chain. C) elongation...

Genetics Practice Exam 55 Questions with Verified Answers Which process is represented by the arrow labeled W? A. replication B. translation C. transcription D. RNA splicing - CORRECT ANSWER C Which of the labeled structures contains RNA with a catalytic function? A. 1 B. 2 C. 4 D. 5 E. 2, 3 and 4 - CORRECT ANSWER C Which of the labeled is made of chromatin? A. 1 B. 1 and 4 C. 4 D. 5 - CORRECT ANSWER A Which of the following molecules, NOT represented on this diagram, a...

AMCB QUESTIONS AND ANSWERS To verify DNA purity we have to: - correct answer Measure absorbance at 260 nm and 280 nm. The addition of proteinase K in the buffer ATL is to: - correct answer Disrupt the cell envelope to lyse cells. The genomic DNA that we extract from cells (of any kind) is polar and sticky. This property is useful for purification purposes. Which of the following surfaces is likely to be used, with good efficiency, to purify DNA. - correct answer A glass rod/bar To qu...

Genetics practice exam 2|42 Questions with Verified Answers . Which enzyme untwists the parent strands of DNA during replication? - CORRECT ANSWER DNA helicase Tetrad analysis is a technique for - CORRECT ANSWER Mapping genes in organisms retaining the four meiotic products in one structure The enzymes most directly concerned with catalyzing DNA synthesis is - CORRECT ANSWER DNA polymerase What did Lederberg and Tatum's 1946-experiment show? - CORRECT ANSWER That sexual recombinatio...