Histotechnology fixation Study guides, Class notes & Summaries

Histotechnology - FixationThe primary purpose of fixation is to: a. stop enzymatic action b. remove the water from the tissue c. provide support for the tissue d. autolyze the tissue - correct answers a Which of the following fixatives contains picric acid, formalin, and acetic acid? a. Zenker b. Helly c. Bouin d. Zamboni - correct answers c The volume of fixative should exceed the volume of the tissue by: a. 1 time to 2 times b. 5 times to 10 times c. 15 times to 20 ...

Histotechnology - Tissue FixationAdditive fixatives Mercuric chloride Chromic acid Picric acid Formaldehyde Glutaraldehyde Osmium tetroxide Potassium dichromate Glyoxal Zinc salts Nonadditive fixatives Alcohols Acetone Acetic Acid Coagulant Fixatives Mercuric acid Chromic acid Picric acid Zinc salts Alcohols Acetone Acetic acid Noncoagulant Fixatives Formaldehyde Glutaraldehyde Osmium Tetroxide Potassium Dichromate Glyoxal Are coagulant fixatives addit...

BOC Study Guide Histotechnology 2nd ed.- Fixation (HT) – Questions/Answers

BOC Study Guide Histotechnology 2nd ed.- Fixation (HT)An example of an additive fixative is one that contains: - correct answers picric acid Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following: - correct answers incomplete fixation Microscopic evalution of H&E stained sections from a surgically removed small bowl specimen shows an absence of much of the epithelium in othe...

T/F To be characterized as a profession, considerable specialized training must be required. CORRECT ANSWER-T Histology is the study of CORRECT ANSWER-tissue T/F The purpose of having a procedure for specimen receipt is to monitor the specimen and the accompanying paperwork. CORRECT ANSWER-T T/F Accessioning is giving a specimen a common number, one that is not unique to the patient. CORRECT ANSWER-f T/F The tasks performed by the histotechnologist require patience, mechanical abilit...

Histotechnology – Fixation – Q’s & A’s

Histotechnology - FixationWhat are the steps tissue must go through before it is viewed under a microscope? - correct answers 1. Fixation 2. Processing 3. Embedding 4. Microtomy 5. Staining 6. Coverslipping Define fixation. - correct answers The preservation of cells and tissue in as life-like a way as possible by stabilizing the protein so that it is resistant to further changes. What are the functions of fixation? - correct answers 1. To prevent putrefaction and autolysis 2. To hel...

Histotechnology – Fixation – Qs/As

Carsons Mordant . substance’s or metals that act as a link between dye and tissue A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. Fixative additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combines with fixative molecule becoming insoluble non additive ...

Histotechnology Practicum: Test 1Biopsy or Gross Specimen: small piece of tissue and derived from simple-natured procedure - correct answers biopsy biopsy or Gross specimen: whole organ and typically derived from surgeries - correct answers gross specimen What are the 10 steps in the pathology lab workflow? - correct answers 1. fixation 2. accession 3. grossing 4. continue fixation 5. dehydration 6. clearing 7. infiltration 8. blocking 9. section 10. staining what is the most i...