Methylation Study guides, Class notes & Summaries

Give two examples of how eukaryotic organisms derive benefits from regulating gene expression - 1) Rabbits change from brown to white due to a response to environmental changes 2) larval to adult is totally different b/c of different proteins during life changes gene expression control is used in multicellular organisms to. . . - define tissues and cells w/ specific functions w/in organism What is the order of regulation of gene expression - transcription, RNA processing, translation, post...

When explaining genetic coding to a group of students, the instructor discusses gene activation and deactivation. It was stressed that inactivation of a gene requires which of the following processes? Correct Answer-Methylation of histone amino acid When an infant is born with gene mutations in his cells, the nurse explains to the parents that accidental errors may be a result of: Select all that apply. Correct Answer-Loss of one or more base pairs Substitution of one base pair for another ...

Describe the five general procedures in DNA cloning Correct Answer-1. Obtaining the DNA segment to be cloned 2. Selecting a small molecule of DNA capable of autonomous replication 3. Joining two DNA fragments covalently 4. Moving recombinant DNA from the test tube to a host organism 5. Selecting or identifying host cells that contain recombinant DNA Are restriction enzymes made by eukaryotes or prokaryotes? What is their function in vivo? Correct Answer-Restriction endonucleases are found...

Angelman Syndrome causes (expression and % of total) correct answers **paternal imprinting defect, should be maternal expression 5-7 kb deletion on maternal chr15q11.2-13 (60-70%) UBE3A maternal deletion (11%) Paternal UPD15 (3-7%) Angelman testing strategy and condition features correct answers methylation first since finds ~80% of cases then UBE3A seq and del/dup features: happy demeanor, abnormal gait, seizures, delayed/absent speech Prader-Willi Syndrome causes (expression and...

ASCP MB: Extra-Practice Questions & Answers 2024 The Klenow fragment of E. coli DNA polymerase I is used: - ANSWER-to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - ANSWER-gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucle...

USMLE Step 1- Biochemistry Questions and Answers(2022)Answered test Histones are rich in which 2 amino acids? Ans- Lysine Arginine What histone protein is responsible for binding to the nucleosome and to the "linker" DNA thereby stabilizing the chromatin fiber? Ans- H1 When does DNA and histone synthesis occur in the cell cycle? Ans- S-phase Will the following be increased or decreased or neutral in heterochromatin? methylation acetylation Ans- methylation increased acety...

USMLE Step 1- Biochemistry Exam Questions & Answers 2023/2024 Histones are rich in which 2 amino acids? - ANSWER-Lysine Arginine What histone protein is responsible for binding to the nucleosome and to the "linker" DNA thereby stabilizing the chromatin fiber? - ANSWER-H1 When does DNA and histone synthesis occur in the cell cycle? - ANSWER-S-phase Will the following be increased or decreased or neutral in heterochromatin? methylation acetylation - ANSWER-methylation increased ...

CBSE FINAL EXAM 2023/2024 GRADED A LATEST VERSION A 66-year-old woman comes to the physician because of severe lower back pain. She is otherwise healthy with well-controlled hypertension and hyperlipidemia. She does not drink, but has smoked one pack of cigarettes per day for the past 20 years. An x-ray of the lumbar spine shows an anterior wedge compression fracture of L1. Results of dual-energy X-ray absorptiometry (DEXA) scan shows substantially decreased bone density in the region...

USMLE Biochemistry Exam Questions with Correct Answers Heterochromatin - ANSWER Highly Condensed DNA Transcriptionally inactive Ex. Barr bodies (inactivated X chromosomes) Euchromatin - ANSWER Less condensed DNA Transcriptionally active DNA Methylation - ANSWER Occurs at *CpG Islands* to repress transcription 'CpG Methylation Makes DNA MUTE' Template strand *cytosine* and *adenine* are methylated, which allow mismatch repair enzymes to distingu...

The tryptophan operon is a repressible operon that is: - ANSWER-Turned off whenever tryptophan is added to the growth medium. The lactose operon is likely to be transcribed when: - ANSWER-The cyclic AMP and lactose levels are both high within the cell. For a repressible operon to be transcribed, which of the following must occur? - ANSWER-RNA polymerase must bind to the promoter, and the repressor must be inactive. Which of the following, when taken up by the cell, binds to the represso...