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Molecular Biology Final Exam Review Questions and Answers
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Eukaryotic pre-mRNAs undergo three processing steps to become mRNA. What are 
they? 1. 5' cap addition 
2. 3' poly-A tail addition 
3. Splicing 
What stimulates resting B-cells to become plasma cells, divide and secrete antibody? 
Finding its target/binding of antigen to the antibody displayed on its surface 
How many different polypeptide chains are in a IgG-antibody Two (two copies of 
heavy chain & two copies of light chain) 
If a heavy chain gene has 50 V segments, 25 D segments, and 6...
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Molecular Biology Comprehensive Final Exam Questions and Answers
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Molecular biology The study of the molecular basis of genes and gene expression; 
molecular genetics. 
Central Dogma theory that states that, in cells, information only flows from DNA to 
RNA to proteins 
Transcription synthesis of an RNA molecule from a DNA template 
Translation Process by which mrna is decoded and a protein is produced 
Replication process of copying DNA prior to cell division
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Molecular Biology Final Exam Practice Queries and Answers
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Alternative RNA splicing a post-transcriptional gene regulation mechanism in 
eukaryotes in which multiple protein products are produced by a single gene through 
alternative splicing combinations of the RNA transcript 
Codon three consecutive nucleotides in mrna that specify the addition of a specific 
amino acid or the release of a polypeptide chain during translation 
DNA ligase the enzyme that catalyzes the joining of DNA fragments together 
DNA polymerase an enzyme that synthesizes a new...
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Molecular Biology Final Exam Study Guide with Questions and Answers
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In the 1944 experiment by Avery et al., only ___ was able to transform a nonvirulent strain of 
bacteria into a virulent one, thereby demonstration that it is likely the ___. 
A) protein; chromosomal material 
B) genetics; protein material 
C) amino acid; DNA material 
D) DNA; genetic material D) DNA; genetic material 
Hershey and Chase (1952) blender experiment demonstrated that 
A) protein is not required for virus production 
B) viral coat proteins can enter the infected bacterial cells 
C) ...
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Molecular Biology Midterm Exam Questions and Answers
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In the double helix structure of nucleic acids, cytosine hydrogen bonds to 
a. deoxyribose 
b. guanine 
c. ribose 
d adenine 
e. thymine b 
Which of the following statements regarding enzyme function is false? 
a. Enzymes are very specific for certain substrates. 
b. An enzyme's function depends on its three-dimensional shape. 
c. Enzymes are used up in chemical reactions. 
d. An enzyme binds to its substrate at the enzyme's active site. 
e. Enzymes emerge unchanged from the reactions they ca...
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Molecular Biology Practice Test Questions and Answers
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Griffith Experiment observed change in streptococcus pneumoniae from bacteria 
without capsules (avirulent R rough colonies) to bacteria that had capsules (virulent S 
smooth colonies); result: heat-killed virulent bacteria could transform avirulent bacteria 
into virulent bacteria (prove that DNA is a genetic element) 
Meselson and Stahl Experiment DNA replicaition was semiconservative 
Avery Experiment genes are made up of DNA 
what are the base-pairing rules? T and A, C and G 
how many hyd...
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Molecular Biology Study Guide with Questions and Answers
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What bases pair together in DNA and RNA? DNA: A&T - C&G 
RNA: A&U - C&G 
What is the shape of DNA? shape - double helix 
Backbone - sugars and phosphates 
Rungs - base pairs 
Who discovered the shape in DNA? What year? james watson and francis clark 
(1953) 
Three types of RNA: mrna (messenger) - single strand, brings info from DNA to 
ribosome 
Rrna (ribosomal) - part of ribosome 
Rrna (transfer) - brings correct amino acid to ribosome to build polypeptide (protein) 
What are three types of ...
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In-depth Molecular Biology Questions and Answers 2023
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Endonuclease A nuclease that breaks the DNA in the middle. 
Exonuclease A nuclease that breaks the DNA up from the ends. 
DNAse An enzyme that breaks down DNA. 
Helicase An enzyme that unwinds and unzips the DNA. 
Hind III A restriction enzyme the cuts up Lambda DNA. 
Klenow Fragment The sections that are being added to by DNA Polymerase III.
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Molecular Biology Test Review Questions and Answers
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DNA and RNA are polymers called_, and are made of monomers called __ nucleic 
acids; amino acids 
What are the three parts of a nucleotide? nitrogen base, phosphate group, sugar 
What does the pentagon, indicated by the number 1 on the figure, represent? sugar 
What does the circle, indicated by the number 2 on the figure, represent? phosphate 
What do the numbers 3 and 4 on the figure represent? nitrogen base 
What type of sugar is found in DNA? deoxyribose
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Molecular Biology Exam Revision Questions and Answers
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Which of the following can be described as the smallest living unit? 
(a)DNA 
(b)cell 
(c)organelle 
(d)protein B. CELL 
The flow of genetic information is controlled by a series of biochemical reactions that 
result in the production of 
proteins, each with its own specific order of amino acids. Choose the correct series 
biochemical reactions from the options presented here. 
(a)replication, transcription, translation (b)replication, translation, transcription 
(c)translation, transcription,...