CRISPR Study guides, Class notes & Summaries

CRISPR Cas-9 Semester 1 Questions With Correct Actual Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM De...

CMMB 403 final Exam New Year 2024| Questions and Answers| Certified Quality Graded A+ what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 1. non-homologous end jo...

What are schedule 1, 2, 3 ,4, 5 drugs? Schedule 1 - no known use (e.g., heroin) Schedule 2 - very addictive (morphine, cocaine) Schedule 3 to 5 - less addictive. What are dangerous drugs? Prescription drugs other than schedule 1-5. How many DEA registrations do you need if you prescribe drugs? Dispense drugs? One to prescribe; a separate registration for each location where you dispense. How often do you renew your DEA license? Every 3 years. Can you move your o...

HC 7- DNA Pair & Genome Editing Questions & Answers 1 / 3 1. What are causes for gene knockout? Non-homologous end joining 2. What is gene knockout? premature stop coden: when the function of a gene has been disrupted or turned off to study gene function 3. What are repair mechanism for DSB? Non-homologeus end joint (NHEJ) + Homologues recombination 4. Where stands CRISPR for? Clustered Regularly Interspaced Short Palindromic Repeats 5. Working mechanism of CRISPR? he palindromic ...

This summary consists of: 1. epigenetic principles underlying gene expression regulation 2. epigenetic inheritance underlying cell type identity 3. differentiation principles, in particular neuronal development 4. genetic and epigenetic editing principles, including CRISPR/Cas platforms 5. critique and debate current trends and challenges in genetic and epigenetic editing principles on an academic level

CRISPR-Cas9 Final Practice Exam Questions 2024.What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kinds of re...

Genetic Analysis Exam 1 Questions & Answers 2024 Genetic Tools - ANSWER-Forward genetics Reverse Genetics Direct expression systems Genomic approaches Forward Genetics - ANSWER-Damage DNA without knowing what damage would be: shotgun 1. mutagenesis with chemical mutagens 2. mutagenesis with x rays 3. transposon insertion mutagenesis Reverse Genetics - ANSWER-Damage specific sites 'targeted' 1. targeted mutageneissi in mice 2. targeted mutageneisis in fly 3. semi-targe...

Jurisprudence Essay - Critical Race Theory

HC 7- DNA Pair & Genome Editing Questions & Answers 1 / 3 1. What are causes for gene knockout? Non-homologous end joining 2. What is gene knockout? premature stop coden: when the function of a gene has been disrupted or turned off to study gene function 3. What are repair mechanism for DSB? Non-homologeus end joint (NHEJ) + Homologues recombination 4. Where stands CRISPR for? Clustered Regularly Interspaced Short Palindromic Repeats 5. Working mechanism of CRISPR? he palindromic ...

CRISPR-Cas9 Complete Study Guide Practice Exam 2024. What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kin...