Sds-page Study guides, Class notes & Summaries

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light? - Condenser lens The magnification of a microscope is equal to.... - the product of magnification of the ocular times the objective lens The absorbance of UV or visible light by a compound and the subsequent release by that compound of some of the energy as longer wavelength (usually visible) light is known as - fluorescen...

Which of the following is an example of a product easily found in nature? - Answer- Antibiotic In a biotechnology lab, tap water is best used for - Answer- washing glassware The order of amino acids on a polypeptide chain is encoded by - Answer- mRNA How do DNA molecules vary from one species to another? - Answer- Number of genes and non-coding regions What is agar? - Answer- Solid media on which bacteria grow What part of an amino acid differs from one to the other? - Answer- R g...

BBMB-301 Test questions and Answers for Final studying (Iowa State) Which class of biopolymers is responsible for separating distinct aqueous compartments within Eukaryotic cells? Lipids The ______ is surrounded by a double membrane and produces approximately 90% of the energy, in the form of ATP, used by a typical living cell Mitochondrion T or F: The central dogma in biochemistry describes the flow of information from RNA to DNA to polypeptide to functional protein False: its DNA to RN...

What is an example of a product easily found in nature? correct answers Antibiotic In a biotechnology lab, what is tap water is best used for? correct answers Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? correct answers mRNA How do DNA molecules vary from one species to another? correct answers Number of genes and non-coding regions. What is agar? correct answers Solid media on which bacteria grow. What part of an amino acid differs from o...

What is biotin and digoxigenin most commonly used for in the lab? Why are they safer? Probe labeling (aka tagging nucleic acids so that they can be visualized). They are safer because they are nonradioactive. Name the three basic methods used to label a DNA probe: 1. End Labeling 2. Nick Translation 3. Random priming What is SDS? Name 3 of its applications in the lab. Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used in SDS-PAGE to denature proteins so they separat...

Biochemistry Test 1 Questions and Answers Graded A+ biochemistry a discipline in biochemistry; is the description of molecules in biology/chemistry of proteins molecular biology a discipline in biochemistry; the manipulation of DNA, genetics cell biology a discipline in biochemistry; larger scale, functions and mechanisms within a cell/cell energetics cellulose a natural polymer; found in plants for storage glycogen a natural polymer; found in animals for storage protein a natural polymer; ...

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS Henderson-Hasselbach Equation - answerpH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - answerUsed in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - answerChanges soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - answerSeparates sample b...

MMSC490 Practice Exam 1|123 Questions with Verified Answers cytoplasmic organelles are: a. absent in prokaryotic cells and present in eukaryotic cells. b. present in both prokaryotic and eukaryotic cells. c. present in prokaryotic cells and absent in eukaryotic cells. d. absent in both prokaryotic and eukaryotic cells. - CORRECT ANSWER a. absent in prokaryotic cells and present in eukaryotic cells E. coli is a useful model system for molecular biology studies because: a. it has a smal...

ACS BIOCHEMISTRY EXAM QUESTIONS WITH CORRECT SOLUTIONS Henderson-Hasselbach Equation - CORRECT ANSWERS pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWERS Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - CORRECT ANSWERS Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in ...